G duodenalis, Diplomonád
Description Summary: Giardia duodenalis is an enteric parasite commonly detected in children. Exposure to this organism may lead to asymptomatic or symptomatic infection.
Additionally, early-life infections by this protozoan have been associated with impaired growth and cognitive function in poor resource settings. However, no molecular investigation was conducted to ascertain the molecular variability of the parasite. Therefore, we describe here the frequency and genetic diversity of G. Genomic DNA from G. No association between the presence of a given assemblage and the occurrence of diarrhoea could be demonstrated.
A total of samples were further analysed by a multi-locus sequence genotyping MLSG approach at the glutamate dehydrogenase gdhß-giardin bg and triose phosphate isomerase tpi genes. Assemblage A sequences were assigned to sub-assemblages AI 0. This study demonstrated that the occurrence of gastrointestinal illness diarrhoea was not associated to a given genotype of G duodenalis.
The assemblage B of the parasite was responsible for nine out of ten infections detected in this paediatric population.
The extremely high genetic diversity observed within assemblage B isolates was compatible with an hyperendemic epidemiological scenario where infections and reinfections were common.
The obtained molecular data may be indicative of high coinfection g duodenalis by different G. Author summary: Giardia duodenalis is a globally distributed enteric parasite, and the most detected intestinal protozoan in children.
Infection by this pathogen can evolve from asymptomatic féreg toxikózis chronic disease with a range of gastrointestinal manifestations including diarrhoea.
In endemic areas, early infection in childhood can contribute to impaired growth and cognitive function. This organism is currently divided in 8 distinct A-H genetic groups assemblages with marked differences in host range and specificity and even geographical distribution.
Therefore, molecular studies aiming at investigating g duodenalis genetic diversity of the parasite are cornerstone to assess its transmission dynamics.
By using targeted-molecular genotyping methods based on PCR and sequencing, we demonstrated here that i the occurrence of g duodenalis in Mozambican young children infected with G. This study represents the largest and most comprehensive molecular investigation evaluating the genetic g duodenalis of G.
In conjunction with previous risk assessment analyses carried out in the country, the data presented here highlights the need g duodenalis additional population genetic and epidemiological studies to characterise the epidemiology and transmission dynamics of G.
Of particular interest would be the investigation of g duodenalis parasite in environmental water samples and in domestic animal species susceptible of acting as suitable reservoirs of human infections.
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